Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- PA1-12361 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ErbB2 (HER-2) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- A suggested positive control for this product is breast carcinoma lysate.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 250 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of SK-BR-3 (Lane 1), T-47D (Lane 2) and MDA-MB-231 (Lane 3). The blots were probed with Anti-ErbB2 Sheep Polyclonal Antibody (Product # PA1-12361, 5 µg/mL) and detected by chemiluminescence using Rabbit anti-Sheep IgG (H+L) Secondary Antibody, HRP conjugate (Product # 61-8620, 0.25 µg/mL, 1:4000 dilution). A 185 kDa band corresponding to ErbB2 was observed in SK-BR-3 and T-47D but not in MDA-MB-231, which is a negative control for ErbB2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of SK-BR-3 (Lane 1), T-47D (Lane 2) and MDA-MB-231 (Lane 3). The blots were probed with Anti-ErbB2 Sheep Polyclonal Antibody (Product # PA1-12361, 5 µg/mL) and detected by chemiluminescence using Rabbit anti-Sheep IgG (H+L) Secondary Antibody, HRP conjugate (Product # 61-8620, 0.25 µg/mL, 1:4000 dilution). A 185 kDa band corresponding to ErbB2 was observed in SK-BR-3 and T-47D but not in MDA-MB-231, which is a negative control for ErbB2.