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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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Validation data
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- Product number
- LS-C796705 - Provider product page
- Provider
- LSBio
- Product name
- ERBB2 / HER2 Antibody (aa676-1255, clone UMAB35) LS-C796705
- Antibody type
- Monoclonal
- Description
- Purified from ascites.
- Reactivity
- Human, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- UMAB35
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of cell lysates of HEK293 transfected with ERBB2 cDNA. (Left lane) or untransfected. (Right lane) by using ERBB2 UltraMAB clone UMAB35, 1:500).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysates. (35ug) from 9 different cell lines. (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of extracts. (35ug) from 9 different cell lines by using anti-ERBB2 monoclonal antibody. (Clone UMAB35) at 1:500.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of mouse tissue lysates. (20ug) from Uterus. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot® Ultra. (1:1000).
