MA5-15702

antibody from Invitrogen Antibodies

Targeting: ERBB2 CD340, HER-2, HER2, NEU, NGL

ELISA

Antibody data

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Validation data

Product number: MA5-15702 - Provider product page
Provider: Invitrogen Antibodies
Product name: ErbB2 (HER-2) Monoclonal Antibody (6C2)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15702 targets ERBB2 in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human samples. The MA5-15702 immunogen is purified recombinant fragment of human ERBB2(aa750-987) expressed in E. Coli. MA5-15702 detects ERBB2 which has a predicted molecular weight of approximately 180kDa.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 6C2
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ERBB2 protein structure - MA5-15702 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ERBB2 using ERBB2 monoclonal antibody (Product # MA5-15702) in SKBR3 (1) and MCF-7 (2) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ErbB2 (HER-2) (Fig. a) was performed by loading 30 µg of SK-BR-3 Control (lane 1), SK-BR-3 ErbB2 knockout (lane 2) whole cell extracts.ErbB2 (HER-2) was detected at 185 kDa using ErbB2 (HER-2) Mouse Monoclonal Antibody (Product # MA5-15702, 1:500 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody HRP conjugate (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal in CRISPR mediated knockout (KO) confirms that antibody is specific to ErbB2 (HER-2).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1) and SK-BR-3 (Lane 2). The blots were probed with Anti-ErbB2 (HER-2) Monoclonal Antibody (Product # MA5-15702, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 185 kDa band corresponding to ErbB2 was observed in SK-BR-3 but not in MDA-MB-231, which is a negative cell line for ErbB2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ERBB2 using ERBB2 monoclonal antibody (Product # MA5-15702) in SKBR3 (1) and MCF-7 (2) cell lysate.