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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- PA5-30968 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UBE2D2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7. Predicted reactivity: Rat (100%), Zebrafish (98%), Xenopus laevis (92%), Pig (98%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Structural insights into the mechanism and E2 specificity of the RBR E3 ubiquitin ligase HHARI.
Yuan L, Lv Z, Atkison JH, Olsen SK
Nature communications 2017 Aug 8;8(1):211
Nature communications 2017 Aug 8;8(1):211
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using UBE2D2 Polyclonal Antibody (Product # PA5-30968). Sample (30 µg of whole cell lysate). Lane A: NT2D1. Lane B: U87-MG . 15% SDS PAGE. UBE2D2 Polyclonal Antibody (Product # PA5-30968) diluted at 1:1,000.
