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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- R20347BTN-0.05ML - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Biotinylated Prostate Specific Antigen Antibody / PSA
- Antibody type
- Monoclonal
- Description
- This highly specific Biotinylated Prostate Specific Antigen antibody is suitable for use in Western blot/Immunohistochemistry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM323
- Vial size
- 50 ul
- Concentration
- Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
- Storage
- Store the recombinant biotinylated Prostate Specific Antigen antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of PSA (purified from human seminal fluid), ACT (alpha 1-antichymotrypsin, from human plasma), and PSA-ACT complex, under non-reduced(-) or reduced(+) conditions, using recombinant Prostate Specific Antigen antibody at 1:2500.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- A titer ELISA of PSA (purified from human seminal fluid). The plate was coated with different amounts of PSA. A serial dilution of recombinant Prostate Specific Antigen antibody was used as the primary. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human prostate cancer tissue with recombinant Prostate Specific Antigen antibody at 1:1000.
