Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF1344 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Kallikrein 3/PSA Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human KLK3 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) KLK2 is observed and less than 5% cross-reactivity with rhKLK1, rhKLK4, rhKLK5, rhKLK7, rhKLK8, rhKLK9, rhKLK10, rhKLK11, rhKLK12, rhKLK13, and rhKLK15 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P07288
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Anatomically-specific intratubular and interstitial biominerals in the human renal medullo-papillary complex.
Cutting edge: permissive MHC class II allele changes the pattern of antitumor immune response resulting in failure of tumor rejection.
Cutting edge: permissive MHC class II allele changes the pattern of antitumor immune response resulting in failure of tumor rejection.
Chen L, Hsi RS, Yang F, Sherer BA, Stoller ML, Ho SP
PloS one 2017;12(11):e0187103
PloS one 2017;12(11):e0187103
Cutting edge: permissive MHC class II allele changes the pattern of antitumor immune response resulting in failure of tumor rejection.
Klyushnenkova EN, Kouiavskaia DV, Berard CA, Alexander RB
Journal of immunology (Baltimore, Md. : 1950) 2009 Feb 1;182(3):1242-6
Journal of immunology (Baltimore, Md. : 1950) 2009 Feb 1;182(3):1242-6
Cutting edge: permissive MHC class II allele changes the pattern of antitumor immune response resulting in failure of tumor rejection.
Klyushnenkova EN, Kouiavskaia DV, Berard CA, Alexander RB
Journal of immunology (Baltimore, Md. : 1950) 2009 Feb 1;182(3):1242-6
Journal of immunology (Baltimore, Md. : 1950) 2009 Feb 1;182(3):1242-6
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Kallikrein 3/PSA by Western Blot. Western blot shows lysates of Recombinant Human Kallikrein 3/PSA (Catalog # 1344-SE), DU145 human prostate carcinoma cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Kallikrein 3/PSA at approximately 28-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Kallikrein 3/PSA by Western Blot. Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Kallikrein 3/PSA at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Kallikrein 3/PSA in LNCaP and MCF-7 Human Cell Lines. Kallikrein 3/PSA was detected in immersion fixed LNCaP human prostate cancer cell line (positive control, left panel) and MCF-7 human breast cancer cell line (negative control, right panel) using Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of LNCaP cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Kallikrein 3/PSA in Human Kidney Cancer Tissue. Kallikrein 3/PSA was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Kallikrein 3/PSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1344) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.