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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- NBP2-29407 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal PMEL17/SILV Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (NKI-beteb) [NBP2-29407] - Formalin-fixed, paraffin-embedded human melanoma tissue stained with gp100 NKI-beteb antibody, peroxidase-conjugate and DAB chromogen.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (NKI-beteb) [NBP2-29407] - Formalin-paraffin human melanoma tissue stained with gp100 Ab (NKI-beteb)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): PMEL17/SILV Antibody (NKI-beteb) [NBP2-29407] - An intracellular stain was performed on SK-MEL-28 cells with PMEL/SILV [NKI-beteb] Antibody NBP2-33172AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image using the Alexa Fluor 647 format of this antibody.
