Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-20753 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NPC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is human kidney tissue lysate. PA5-20753 can be used with blocking peptide PEP-0867.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human kidney tissue lysate using a NPC1 polyclonal antibody (Product # PA5-20753) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NPC1 in HepG2 cell lysate with NPC1 Polyclonal Antibody (Product # PA5-20753) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-NPC1 Polyclonal Antibody (Product # PA5-20753) and a 180 kDa band corresponding to NPC intracellular cholesterol transporter 1 was observed in U-87 MG, Hep G2, Mouse Liver but not in Mouse Skeletal Muscle. Whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), Hep G2 (Lane 2), Mouse Liver (Lane 3), Mouse Skeletal Muscle (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of NPC1 in Mouse Kidney cells with NPC1 Polyclonal Antibody (Product # PA5-20753) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of NPC1 in mouse kidney tissue with NPC1 Polyclonal Antibody (Product # PA5-20753) at 2.5 µg/mL.