Explore
Validate
Learn600-401-351
antibody from Rockland Immunochemicals, Inc.
Targeting: ANAPC10
APC10, DKFZP564L0562, DOC1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 600-401-351 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-351, RRID:AB_2226990
- Product name
- Anti-APC10 (RABBIT) Antibody - 600-401-351
- Antibody type
- Polyclonal
- Vial size
- 100 µl
No comments: Submit comment
Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Affinity Purified Rabbit anti-APC10 was used at a 1:500 dilution to detect human APC10 in various cell extracts. This antibody clearly detects a ~26 kDa band corresponding to human APC10 (predicted molecular weight is 21 kDa). All lanes contain 20 µg of lysate or extract as follows: lane 1, HeLa nuclear extract; lane 2, HeLa whole cell lysate; lane 3, A431 whole cell lysate; lane 4, Jurkat whole cell lysate; lane 5, 293 whole cell lysate. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 4 min.
- Validation comment
- Western Blot
