PA5-53790

antibody from Invitrogen Antibodies

Targeting: RBM8A BOV-1A, BOV-1B, BOV-1C, RBM8, RBM8B, Y14, ZNRP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-53790

Provider

Invitrogen Antibodies

Product name

RBM8A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Immunogen sequence: MADVLDLHEA GGEDFAMDED GDESIHKLKE KAKKRKGRGF GSEEGSRARM REDYDSVEQD GDEPGPQRSV EGWILFV Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Prognostic value and oncogenic effect of increased RBM8A expression in colon adenocarcinoma.

Biochemistry and cell biology = Biochimie et biologie cellulaire 2021 Oct;99(5):x

---

The exon-junction complex helicase eIF4A3 controls cell fate via coordinated regulation of ribosome biogenesis and translational output.

Kanellis DC, Espinoza JA, Zisi A, Sakkas E, Bartkova J, Katsori AM, Boström J, Dyrskjøt L, Broholm H, Altun M, Elsässer SJ, Lindström MS, Bartek J
Science advances 2021 Aug;7(32)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RBM8A in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a RBM8A Polyclonal Antibody (Product # PA5-53790). Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of RBM8A in human cell line A-431 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a RBM8A Polyclonal Antibody (Product # PA5-53790).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorecent analysis of RBM8A in human cell line A-431 using RBM8A Polyclonal Antibody (Product # PA5-53790). Staining shows localization to nuclear speckles.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RBM8A in human lymph node using RBM8A Polyclonal Antibody (Product # PA5-53790) shows strong nuclear positivity in germinal center cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RBM8A in human skeletal muscle using RBM8A Polyclonal Antibody (Product # PA5-53790) shows strong nuclear positivity in myocytes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RBM8A in human skin using RBM8A Polyclonal Antibody (Product # PA5-53790) shows strong nuclear positivity in squamous epithelial cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RBM8A in human small intestine using RBM8A Polyclonal Antibody (Product # PA5-53790) shows strong nuclear positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RBM8A in human skeletal muscle using RBM8A Polyclonal Antibody (Product # PA5-53790) shows strong nuclear positivity in myocytes.