NB100-56118

antibody from Novus Biologicals

Targeting: CASP9 APAF-3, ICE-LAP6, MCH6, PPP1R56

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-56118

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-56118, RRID:AB_837884

Product name

Rabbit Polyclonal Caspase-9 Antibody

Antibody type

Polyclonal

Description

Unpurified. This antibody detects both the pro- and active-Caspase 9 forms. In western blots, the proform of caspase-9 is detected at ~50 kDa, the large subunit at ~35 kDa, and the small subunit at ~15 kDa. Intermediate caspase-9 cleavage forms may also be seen at ~21 kDa.

Reactivity

Human, Mouse, Rat, Canine

Host

Rabbit

Isotype

IgG

Vial size

0.05 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Effects of neoadjuvant FOLFIRINOX and gemcitabine-based chemotherapy on cancer cell survival and death in patients with pancreatic ductal adenocarcinoma.

Xie L, Xia L, Klaiber U, Sachsenmaier M, Hinz U, Bergmann F, Strobel O, Büchler MW, Neoptolemos JP, Fortunato F, Hackert T
Oncotarget 2019 Dec 31;10(68):7276-7287

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VEGFC/VEGFR3 Signaling Regulates Mouse Spermatogonial Cell Proliferation via the Activation of AKT/MAPK and Cyclin D1 Pathway and Mediates the Apoptosis by affecting Caspase 3/9 and Bcl-2.

Zhao L, Zhu Z, Yao C, Huang Y, Zhi E, Chen H, Tian R, Li P, Yuan Q, Xue Y, Wan Z, Yang C, Gong Y, He Z, Li Z
Cell cycle (Georgetown, Tex.) 2018;17(2):225-239

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RIP3 attenuates the pancreatic damage induced by deletion of ATG7.

Zhou X, Xie L, Xia L, Bergmann F, Büchler MW, Kroemer G, Hackert T, Fortunato F
Cell death & disease 2017 Jul 13;8(7):e2918

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Skin Diseases in Laboratory Mice: Approaches to Drug Target Identification and Efficacy Screening.

Sundberg JP, Silva KA, King LE Jr, Pratt CH
Methods in molecular biology (Clifton, N.J.) 2016;1438:199-224

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New Strategy That Delays Progression of Amyotrophic Lateral Sclerosis in G1H-G93A Transgenic Mice: Oral Administration of Xanthine Oxidoreductase Inhibitors That Are Not Substrates for the Purine Salvage Pathway.

Kato S, Kato M, Kusano T, Nishino T
Journal of neuropathology and experimental neurology 2016 Dec 1;75(12):1124-1144

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The Effects of Difumarate Salt S-15176 after Spinal Cord Injury in Rats.

Erdoğan H, Tunçdemir M, Kelten B, Akdemir O, Karaoğlan A, Taşdemiroğlu E
Journal of Korean Neurosurgical Society 2015 Jun;57(6):445-54

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Chronic proliferative dermatitis in Sharpin null mice: development of an autoinflammatory disease in the absence of B and T lymphocytes and IL4/IL13 signaling.

Potter CS, Wang Z, Silva KA, Kennedy VE, Stearns TM, Burzenski L, Shultz LD, Hogenesch H, Sundberg JP
PloS one 2014;9(1):e85666

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Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells.

Pradhan N, Pratheek BM, Garai A, Kumar A, Meena VS, Ghosh S, Singh S, Kumari S, Chandrashekar TK, Goswami C, Chattopadhyay S, Kar S, Maiti PK
Cell biology international 2014 Oct;38(10):1118-31

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Upregulation of miR-18a-5p contributes to epidermal necrolysis in severe drug eruptions.

Ichihara A, Wang Z, Jinnin M, Izuno Y, Shimozono N, Yamane K, Fujisawa A, Moriya C, Fukushima S, Inoue Y, Ihn H
The Journal of allergy and clinical immunology 2014 Apr;133(4):1065-74

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The effects of lornoxicam on neuroprotection following diffuse traumatic brain injury in rats.

Topcu I, Vatansever S, Bayram E, Var A, Cetin I, Civi M
Turkish neurosurgery 2013;23(6):764-71

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Apoptosis in extracorporeal preserved inguinal fat flaps of the rat.

Herold C, Rennekampff HO, Ohm L, Strauss S, Linkner J, Reimers K, Allmeling C, Vaske B, Vogt PM
Apoptosis : an international journal on programmed cell death 2012 Apr;17(4):400-9

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Interferon-γ exacerbates dry eye-induced apoptosis in conjunctiva through dual apoptotic pathways.

Zhang X, Chen W, De Paiva CS, Corrales RM, Volpe EA, McClellan AJ, Farley WJ, Li DQ, Pflugfelder SC
Investigative ophthalmology & visual science 2011 Aug 9;52(9):6279-85

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Cycling exercise affects the expression of apoptosis-associated microRNAs after spinal cord injury in rats.

Liu G, Keeler BE, Zhukareva V, Houlé JD
Experimental neurology 2010 Nov;226(1):200-6

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Effect of continuous infusion of asialoerythropoietin on short-term changes in infarct volume, penumbra apoptosis and behaviour following middle cerebral artery occlusion in rats.

Price CD, Yang Z, Karlnoski R, Kumar D, Chaparro R, Camporesi EM
Clinical and experimental pharmacology & physiology 2010 Feb;37(2):185-92

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Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation.

Krajewska M, Rosenthal RE, Mikolajczyk J, Stennicke HR, Wiesenthal T, Mai J, Naito M, Salvesen GS, Reed JC, Fiskum G, Krajewski S
Experimental neurology 2004 Oct;189(2):261-79

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Specific caspase interactions and amplification are involved in selective neuronal vulnerability in Huntington's disease.

Hermel E, Gafni J, Propp SS, Leavitt BR, Wellington CL, Young JE, Hackam AS, Logvinova AV, Peel AL, Chen SF, Hook V, Singaraja R, Krajewski S, Goldsmith PC, Ellerby HM, Hayden MR, Bredesen DE, Ellerby LM
Cell death and differentiation 2004 Apr;11(4):424-38

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Caspases 3 and 9 send a pro-apoptotic signal from synapse to cell body in olfactory receptor neurons.

Cowan CM, Thai J, Krajewski S, Reed JC, Nicholson DW, Kaufmann SH, Roskams AJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2001 Sep 15;21(18):7099-109

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Release of caspase-9 from mitochondria during neuronal apoptosis and cerebral ischemia.

Krajewski S, Krajewska M, Ellerby LM, Welsh K, Xie Z, Deveraux QL, Salvesen GS, Bredesen DE, Rosenthal RE, Fiskum G, Reed JC
Proceedings of the National Academy of Sciences of the United States of America 1999 May 11;96(10):5752-7

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Release of caspase-9 from mitochondria during neuronal apoptosis and cerebral ischemia.

Krajewski S, Krajewska M, Ellerby LM, Welsh K, Xie Z, Deveraux QL, Salvesen GS, Bredesen DE, Rosenthal RE, Fiskum G, Reed JC
Proceedings of the National Academy of Sciences of the United States of America 1999 May 11;96(10):5752-7

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Capase-9 in mouse transplanted lung tumor section using anti-Caspase-9 antibody. Image from verified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis Human Brain sections stained for cleaved Caspase-9 expression using this antibody at 1:2000. A) Section from a patient 24 hr after head trauma. B) Control: section from a patient with no known neurological disease or head injury. Hematoxylin-eosin counterstain.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Dog Brain sections stained for cleaved Caspase-9 expression using this antibody at 1:2000. A) Section from a dog brain 2 hr after reperfusion injury. B) ection from a dog brain sham control (brain surgery but no injury). Hematoxylin-eosin counterstain.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Dog Brain sections stained for Active/Cleaved Caspase-9 expression at 1:2000. A and B. The pattern of Caspase-9 staining may vary between different types of neurons. Hematoxylin-eosin counterstain.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Exacerbated apoptosis and altered inflammation in double-deficient Atg7deltapan-Rip3-/- mice. (c) Depletion of Rip3 and pancreatic Atg7 (Atg7deltapan-Rip3d/d) increased pancreatic caspase-9 in 12-week-old mice. Caspase-9 quantitation and representative IF microphotographs of Atg7deltapan (n=5) and Atg7deltapan-Rip3d/d (n=5) pancreatic tissue stained for DAPI (blue) and Caspase-9 (red) (1/1000).. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/cddis.2017.313), licensed under a CC-BY licence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Autophagy-deficient mice showed increased activity of apoptosis and necroptosis. Increased the expression of Caspase-9 in 12-week-old Atg7deltapan mice. Caspase-9 quantitation and representative IF microphotographs of Atg7F/F (n=5) and Atg7deltapan (n=5) pancreatic tissue stained for DAPI (blue) and Caspase-9 (green) (1/1000, scale bar=50?um. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/cddis.2017.313), licensed under a CC-BY licence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Mice with two different spontaneous mutant alleles of Sharpin show variations in onset of CPDM phenotype. However, the onset is much more rapid in Sharpincpdm-Dem mice as evidenced by antibody detection of cleaved CASPASE 9 (T) when compared to Sharpincpdm mutants (R) or WT mice (Q,S). . Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0085666) licensed under a CC-BY licence.