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Proximity ligation assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Proximity ligation assay [2]
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Validation data
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- Product number
- H00000842-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000842-M02, RRID:AB_606024
- Product name
- CASP9 monoclonal antibody (M02), clone 4F12-G6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant CASP9.
- Antigen sequence
MDEADRRLLRRCRLRLVEELQVDQLWDALLSRELF
RPHMIEDIQRAGSGSRRDQARQLIIDLETRGSQAL
PLFISCLEDTGQDMLASFLRTNRQAAKLSKPTLEN
LTPVVLRPEIRKPEVLRPETPRPVDIGSGGFGDVG
ALESLRGNADLAYILSMEPCGHCLIINNVNFCRES
GLRTRTGSNIDCEKLRRRFSSLHFMVEVKGDLTAK
KMVLALLELAQQDHGALDCCVVVILSHGCQASHLQ
FPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPK
LFFIQACGGEQKDHGFEVASTSPEDESPGSNPEPD
ATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPG
FVSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLL
RVANAVSVKGIYKQMPGCFNFLRKKLFFKTS- Isotype
- IgG
- Antibody clone number
- 4F12-G6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between MAPK3 and CASP9. Huh7 cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-CASP9 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between BCL2L1 and CASP9. HeLa cells were stained with anti-BCL2L1 rabbit purified polyclonal 1:1200 and anti-CASP9 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
