Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB0248 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0248, RRID:AB_1019047
- Product name
- CASP9 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against full length recombinant CASP9.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation.
Krajewska M, Rosenthal RE, Mikolajczyk J, Stennicke HR, Wiesenthal T, Mai J, Naito M, Salvesen GS, Reed JC, Fiskum G, Krajewski S
Experimental neurology 2004 Oct;189(2):261-79
Experimental neurology 2004 Oct;189(2):261-79
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CASP9. Various tissue lysates were prepared from human autopsy material and normalized for total protein. Most tissues contained the ~50 KDa pro-CASP9 protein. Cleaved CASP9 was identified in brain cortex and peripheral nerve tissue samples. Pro-C9 : recombinant human pro-CASP9 (full-length) protein. PRO : pro-CASP9. L : large subunit of cleaved CASP9. S : small subunit of cleaved CASP9.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CASP9 expression in formalin-fixed, paraffin-embedded mouse brain tissue sections. Using CASP9 polyclonal antibody (Cat # PAB0248) at 1 : 2000. Hematoxylin-eosin counterstain. A : Brain striatum. B : Brain stem motor neuron.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)