A00080-4

antibody from Boster Biological Technology

Targeting: CASP9 APAF-3, ICE-LAP6, MCH6, PPP1R56

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A00080-4

Provider

Boster Biological Technology

Product name

Anti-Caspase-9 p35/Casp9 Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for Caspase-9 p35/Casp9 detection. Tested with WB, FCM, Direct ELISA in Mouse;Rat.

Reactivity

Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Regulatory role of oxidative stress in retrorsine - Induced apoptosis and autophagy in primary rat hepatocytes.

Zhu Y, Zhang S, Shao Y, Tang L, Zhang C, Tang S, Lu H
Ecotoxicology and environmental safety 2024 Jul 1;279:116515

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A natural halogenated fluoride adenosine analog 5'-fluorodeoxy adenosine induced anticolon cancer activity in vivo and in vitro.

Ma L, Liu T, Lu Y, Dong Y, Zhao X, Man S
Environmental toxicology 2022 Oct;37(10):2493-2502

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Aconitine induces cell apoptosis via mitochondria and death receptor signaling pathways in hippocampus cell line.

Wang H, Liu Y, Guo Z, Wu K, Zhang Y, Tian Y, Zhao B, Lu H
Research in veterinary science 2022 Mar;143:124-133

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Nuciferine Inhibits TMEM16A in Dietary Adjuvant Therapy for Lung Cancer.

Bai X, Liu X, Li S, An H, Kang X, Guo S
Journal of agricultural and food chemistry 2022 Mar 30;70(12):3687-3696

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Mechanism of Action of Xiaoyao San in Treatment of Ischemic Stroke is Related to Anti-Apoptosis and Activation of PI3K/Akt Pathway.

Xu Y, Chen W, Chen Z, Huang M, Yang F, Zhang Y
Drug design, development and therapy 2021;15:753-767

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Anti-Myocardial Ischemia Reperfusion Injury Mechanism of Dried Ginger-Aconite Decoction Based on Network Pharmacology.

Xie F, Wu YY, Duan GJ, Wang B, Gao F, Wei PF, Chen L, Liu AP, Li M
Frontiers in pharmacology 2021;12:609702

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Protective effects of exogenous β-hydroxybutyrate on paraquat toxicity in rat kidney.

Wei T, Tian W, Liu F, Xie G
Biochemical and biophysical research communications 2014 May 16;447(4):666-71

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of Caspase-9 p35/Casp9 using anti-Caspase-9 p35/Casp9 antibody (A00080-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat skeletal muscle tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-9 p35/Casp9 antigen affinity purified polyclonal antibody (Catalog # A00080-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-9 p35/Casp9 at approximately 37/46KD. The expected band size for Caspase-9 p35/Casp9 is at 37/46KD.

Additional image