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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-56122 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56122, RRID:AB_837882
- Product name
- Rabbit Polyclonal Caspase-9 Antibody
- Antibody type
- Polyclonal
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Caspase-9 [p Ser196] Antibody [NB100-56122] - Analysis of human Jurkat cells were left untreated or were staurosporine-treated, lysed and probed with Caspase- 9 antibody at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caspase-9 [p Ser196] Antibody - Unpurified [NB100-56122] - Caspase-9 [p Ser196] Antibody [NB100-56122] - Human colon cancer using Caspase-9 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Caspase 9 was observed. Staining was performed by Histowiz.
