MA1-16842

APAF-3, ICE-LAP6, MCH6, PPP1R56

Antibody data

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Validation data

Provider	Type	Product Number
- No reagents -

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: The pro and active forms of Caspase 9 detected in active 293 cell lysate (lane 2). Lane 1: inactive 293 cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-431 (Lane 1), U-87 MG (Lane 2), HeLa (Lane 3), HeLa treated with Staurosporine (1uM for 3 hours) (Lane 4), PANC-1 (Lane 5), A549 (Lane 6) and HT-29 (Lane 7). The blot was probed with Anti-Caspase 9 Monoclonal Antibody (Product # MA1-16842, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 46 kDa band corresponding to Caspase 9 was observed across the cell lines tested and a 37 kDa band was also observed corresponding to cleaved Caspase 9.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Caspase 9 was achieved by transfecting HeLa cells with Caspase 9 specific siRNAs (Silencer® select Product # s2429, s2430). Western blot analysis (Fig. a) was performed using whole cell extracts from the Caspase 9 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Caspase 9 Polyclonal Antibody (Product # MA1-16842, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Caspase 9.