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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- MA1-16842 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Caspase 9 Monoclonal Antibody (LAP6 96-2-22)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Suggested positive control: HEK 293 whole cell lysate.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- LAP6 96-2-22
- Vial size
- 200 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Simple and Efficient Protocol for Subcellular Fractionation of Normal and Apoptotic Cells.
The expressions of HSP70 and αB-crystallin in myocarditis associated with foot-and-mouth disease virus in lambs.
The expressions of HSP70 and αB-crystallin in myocarditis associated with foot-and-mouth disease virus in lambs.
Ovarian expression of markers associated with proliferation or apoptosis in women with diminished ovarian reserve.
Senichkin VV, Prokhorova EA, Zhivotovsky B, Kopeina GS
Cells 2021 Apr 9;10(4)
Cells 2021 Apr 9;10(4)
The expressions of HSP70 and αB-crystallin in myocarditis associated with foot-and-mouth disease virus in lambs.
Gulbahar MY, Kabak YB, Karayigit MO, Yarim M, Guvenc T, Parlak U
Journal of veterinary science 2011 Mar;12(1):65-73
Journal of veterinary science 2011 Mar;12(1):65-73
The expressions of HSP70 and αB-crystallin in myocarditis associated with foot-and-mouth disease virus in lambs.
Gulbahar MY, Kabak YB, Karayigit MO, Yarim M, Guvenc T, Parlak U
Journal of veterinary science 2011 Mar;12(1):65-73
Journal of veterinary science 2011 Mar;12(1):65-73
Ovarian expression of markers associated with proliferation or apoptosis in women with diminished ovarian reserve.
Vital-Reyes V, Rodríguez-Burford C, Chhieng DC, Alvarado-Cabrero I, Reyes-Fuentes A, Grizzle WE
Fertility and sterility 2006 Jul;86(1):176-85
Fertility and sterility 2006 Jul;86(1):176-85
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- The pro and active forms of Caspase 9 detected in active 293 cell lysate (lane 2). Lane 1: inactive 293 cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-431 (Lane 1), U-87 MG (Lane 2), HeLa (Lane 3), HeLa treated with Staurosporine (1uM for 3 hours) (Lane 4), PANC-1 (Lane 5), A549 (Lane 6) and HT-29 (Lane 7). The blot was probed with Anti-Caspase 9 Monoclonal Antibody (Product # MA1-16842, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 46 kDa band corresponding to Caspase 9 was observed across the cell lines tested and a 37 kDa band was also observed corresponding to cleaved Caspase 9.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase 9 in 293 cell lysate. Samples were incubated in Caspase 9 monoclonal antibody (Product # MA1-16842). Lane 1: inactive 293 cell lysate; Lane 2: active 293 cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase 9 in Jurkat and HeLa cell lysate. Samples were incubated in Caspase 9 monoclonal antibody (Product # MA1-16842) using a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase 9 in 0.2 mg/mL Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Staurosporine (STS) for 3 hours. Samples were incubated in Caspase 9 monoclonal antibody (Product # MA1-16842) using a dilution of 20 µg/mL. A specific band was detected for Caspase 9 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Caspase 9 was achieved by transfecting HeLa cells with Caspase 9 specific siRNAs (Silencer® select Product # s2429, s2430). Western blot analysis (Fig. a) was performed using whole cell extracts from the Caspase 9 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Caspase 9 Polyclonal Antibody (Product # MA1-16842, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Caspase 9.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Caspase 9 in HeLa cells. Samples were incubated in Caspase 9 monoclonal antibody (Product # MA1-16842) followed by DyLight 488 (Green). Alpha-tubulin and nuclei were counterstained against DyLight 550 (Red) and DAPI (Blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Validation of the L&W protocol for nucleus/cytoplasm fractionation of apoptotic cells. WB analysis of cytoplasmic and nuclear fractions from Caov-4 and HeLa cells treated with 0.1 uM staurosporine (Sts), 10 ng/mL TNF-alpha + 5 mug/mL cycloheximide (TNF + Chx), or 35 uM cisplatin (cis). The purity of the resulting fractions was assessed by staining for markers of the cell membrane (Na/K ATPase), ER (ERp-29), mitochondria (cytochrome c ), nuclear envelope (Lamin B), and nucleoplasm (H2AX). PARP cleavage was evaluated as a marker of cell death. Cyto, cytoplasmic fraction; nucl, nuclear fraction.
