Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-12506 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Caspase 9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for this product is HeLa whole cell lysate.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references FOXA1 knock-out via CRISPR/Cas9 altered Casp-9, Bax, CCND1, CDK4, and fibronectin expressions in LNCaP cells.
Albayrak G, Konac E, Ugras Dikmen A, Bilen CY
Experimental biology and medicine (Maywood, N.J.) 2018 Aug;243(12):990-994
Experimental biology and medicine (Maywood, N.J.) 2018 Aug;243(12):990-994
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Caspase 9 was achieved by transfecting HeLa cells with Caspase 9 specific siRNAs (Silencer® select Product # s2429 & s2430). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the Caspase 9 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-Caspase 9 Polyclonal Antibody (Product # PA1-12506, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Caspase 9.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with Staurosporine (1uM for 3hrs) (Lane 2), Jurkat (Lane 3), PANC-1 (Lane 4) and A549 (Lane 5). The blot was probed with Anti-Caspase 9 Polyclonal Antibody (Product # PA1-12506, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 46 kDa band corresponding to Caspase 9 was observed across the cell lines tested and a 37 kDa band was also observed corresponding to cleaved Caspase 9.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase 9 using a polyclonal antibody (Product # PA1-12506).
