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LearnPA5-19903
CASP9 antibody from Invitrogen Antibodies
APAF-3, ICE-LAP6, MCH6, PPP1R56
Antibody data
- Antibody Data
- References [0]
- Comments [0]
- Validations
- Western blot [11]
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- Product number
- PA5-19903
- Provider
- Invitrogen Antibodies
- Product name
- Caspase 9 Polyclonal Antibody
- Provider product page
- Invitrogen Antibodies - PA5-19903
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- In Western blot applications, this antibody detects a band at ~46kDa. A suggested positive control is Hela cell lysate.
- Concentration
- 1.0 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HeLa whole cell lysate using a Caspase-9 polyclonal antibody (Product # PA5-19903) at 1:1000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in HeLa Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9 Polyclonal Antibody (Product # PA5-19903) (Lane 1: 0.5 µg/mL; Lane 2: 1 µg/mL and Lane 3: 2 µg/mL) , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9 Polyclonal Antibody (Product # PA5-19903) (1 µg/mL)), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human Heart. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9 Polyclonal Antibody (Product # PA5-19903) 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 1 µg/mL Lane 2: 2 µg/mL Lane 3: 4 µg/mL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Mouse Spleen Tissue. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9 Polyclonal Antibody (Product # PA5-19903) (Lane 1: 0.5 µg/mL; Lane 2: 1 µg/mL and Lane 3: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 0.5 µg/mL Lane 2: 1 µg/mL and Lane 3: 2 µg/mL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Rat Spleen Tissue. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9 Polyclonal Antibody (Product # PA5-19903) (Lane 1: 0.5 µg/mL; Lane 2: 1 µg/mL and Lane 3: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in HeLa Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9 Polyclonal Antibody (Product # PA5-19903) (Lane 1: 0.5 µg/mL; Lane 2: 1 µg/mL and Lane 3: 2 µg/mL) , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9 Polyclonal Antibody (Product # PA5-19903) (1 µg/mL)), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human Heart. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9 Polyclonal Antibody (Product # PA5-19903) 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 1 µg/mL Lane 2: 2 µg/mL Lane 3: 4 µg/mL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Mouse Spleen Tissue. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9 Polyclonal Antibody (Product # PA5-19903) (Lane 1: 0.5 µg/mL; Lane 2: 1 µg/mL and Lane 3: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 0.5 µg/mL Lane 2: 1 µg/mL and Lane 3: 2 µg/mL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Rat Spleen Tissue. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9 Polyclonal Antibody (Product # PA5-19903) (Lane 1: 0.5 µg/mL; Lane 2: 1 µg/mL and Lane 3: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
