PA5-19903

antibody from Invitrogen Antibodies

Targeting: CASP9 APAF-3, ICE-LAP6, MCH6, PPP1R56

Antibody data

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Validation data

Product number: PA5-19903 - Provider product page
Provider: Invitrogen Antibodies
Product name: Caspase 9 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: In Western blot applications, this antibody detects a band at ~46kDa. A suggested positive control is Hela cell lysate.
Concentration: 1.0 mg/mL

CASP9 protein structure - PA5-19903 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HeLa whole cell lysate using a Caspase-9 polyclonal antibody (Product # PA5-19903) at 1:1000 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot Validation in HeLa Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9 Polyclonal Antibody (Product # PA5-19903) (Lane 1: 0.5 µg/mL; Lane 2: 1 µg/mL and Lane 3: 2 µg/mL) , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9 Polyclonal Antibody (Product # PA5-19903) (1 µg/mL)), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot Validation in Human Heart. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9 Polyclonal Antibody (Product # PA5-19903) 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 1 µg/mL Lane 2: 2 µg/mL Lane 3: 4 µg/mL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot Validation in Mouse Spleen Tissue. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9 Polyclonal Antibody (Product # PA5-19903) (Lane 1: 0.5 µg/mL; Lane 2: 1 µg/mL and Lane 3: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 0.5 µg/mL Lane 2: 1 µg/mL and Lane 3: 2 µg/mL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot Validation in Rat Spleen Tissue. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9 Polyclonal Antibody (Product # PA5-19903) (Lane 1: 0.5 µg/mL; Lane 2: 1 µg/mL and Lane 3: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.