PA5-19904

APAF-3, ICE-LAP6, MCH6, PPP1R56

Antibody data

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Validation data

Product number: PA5-19904
Provider: Invitrogen Antibodies
Product name: Anti-Caspase 9 Polyclonal Antibody
Provider product page: Invitrogen Antibodies - PA5-19904
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: In Western blot applications, this antibody detects a band at ~46kDa. A suggested positive control is Hela cell lysate. PA5-19904 can be used with blocking peptide PEP-0030.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Provider	Type	Product Number
- No reagents -

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HeLa whole cell lysate using a Caspase-9 polyclonal antibody (Product # PA5-19904) at 1:1000 dilution

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Caspase 9 was achieved by transfecting HeLa cells with Caspase 9 specific siRNAs (Silencer® select Product # s2429, s2430). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the Caspase 9 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-Caspase 9 Polyclonal Antibody (Product # PA5-19904, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Caspase 9.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), HeLa (Lane 2), HeLa treated with Staurosporine (1uM for 3 hours) (Lane 3), PANC-1 (Lane 4) and A549 (Lane 5). The blot was probed with Anti-Caspase 9 Polyclonal Antibody (Product # PA5-19904, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 46 kDa band corresponding to Caspase 9 was observed across the cell lines tested and bands at 39 & 37 kDa was also observed corresponding to cleaved Caspase 9.