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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [4]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-19904 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Caspase 9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- In Western blot applications, this antibody detects a band at ~46 kDa. A suggested positive control is Hela cell lysate.
- Concentration
- 1 mg/mL
Submitted references Synergistic effects of cisplatin and proteasome inhibitor bortezomib on human bladder cancer cells.
Konac E, Varol N, Kiliccioglu I, Bilen CY
Oncology letters 2015 Jul;10(1):560-564
Oncology letters 2015 Jul;10(1):560-564
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HeLa whole cell lysate using a Caspase-9 polyclonal antibody (Product # PA5-19904) at 1:1000 dilution
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), HeLa (Lane 2), HeLa treated with Staurosporine (1uM for 3 hours) (Lane 3), PANC-1 (Lane 4) and A549 (Lane 5). The blot was probed with Anti-Caspase 9 Polyclonal Antibody (Product # PA5-19904, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 46 kDa band corresponding to Caspase 9 was observed across the cell lines tested and bands at 39 & 37 kDa was also observed corresponding to cleaved Caspase 9.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Caspase 9 was achieved by transfecting HeLa cells with Caspase 9 specific siRNAs (Silencer® select Product # s2429, s2430). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the Caspase 9 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-Caspase 9 Polyclonal Antibody (Product # PA5-19904, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Caspase 9.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9 Polyclonal Antibody (Product # PA5-19904) (1 µg/mL)), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Mouse Cell Line. Loading: 15 µg of 3T3/NIH cell lysate. Antibodies: Caspase 9 Polyclonal Antibody (Product # PA5-19904) (1 µg/mL)), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of K562 cells using a Caspase-9 polyclonal antibody (Product # PA5-19904) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of HeLa cells using Caspase 9 Polyclonal Antibody (Product # PA5-19904) at 5 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Caspase 9 with Caspase 9 Polyclonal Antibody (Product # PA5-19904) at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Caspase 9 with Caspase 9 Polyclonal Antibody (Product # PA5-19904) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of K562 cells using a Caspase-9 polyclonal antibody (Product # PA5-19904) at a 2 µg/mL dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
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