Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [7]
- Other assay [1]
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- Product number
- PA5-19904 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Caspase 9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- In Western blot applications, this antibody detects a band at ~46 kDa. A suggested positive control is Hela cell lysate. PA5-19904 can be used with blocking peptide PEP-0030.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Synergistic effects of cisplatin and proteasome inhibitor bortezomib on human bladder cancer cells.
Konac E, Varol N, Kiliccioglu I, Bilen CY
Oncology letters 2015 Jul;10(1):560-564
Oncology letters 2015 Jul;10(1):560-564
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of K562 cells using a Caspase-9 polyclonal antibody (Product # PA5-19904) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of HeLa cells using Caspase 9 Polyclonal Antibody (Product # PA5-19904) at 5 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Caspase 9 with Caspase 9 Polyclonal Antibody (Product # PA5-19904) at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Caspase 9 with Caspase 9 Polyclonal Antibody (Product # PA5-19904) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Caspase 9 with Caspase 9 Polyclonal Antibody (Product # PA5-19904) at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Caspase 9 with Caspase 9 Polyclonal Antibody (Product # PA5-19904) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of HeLa cells using Caspase 9 Polyclonal Antibody (Product # PA5-19904) at 5 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL