PA5-19904

antibody from Invitrogen Antibodies

Targeting: CASP9 APAF-3, ICE-LAP6, MCH6, PPP1R56

Western blot

Immunoprecipitation

Antibody data

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Validation data

Product number: PA5-19904 - Provider product page
Provider: Invitrogen Antibodies
Product name: Caspase 9 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: In Western blot applications, this antibody detects a band at ~46 kDa. A suggested positive control is Hela cell lysate. PA5-19904 can be used with blocking peptide PEP-0030.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

CASP9 protein structure - PA5-19904 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of K562 cells using a Caspase-9 polyclonal antibody (Product # PA5-19904) at a 20 µg/mL dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry of HeLa cells using Caspase 9 Polyclonal Antibody (Product # PA5-19904) at 5 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Caspase 9 with Caspase 9 Polyclonal Antibody (Product # PA5-19904) at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Caspase 9 with Caspase 9 Polyclonal Antibody (Product # PA5-19904) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Caspase 9 with Caspase 9 Polyclonal Antibody (Product # PA5-19904) at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Caspase 9 with Caspase 9 Polyclonal Antibody (Product # PA5-19904) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry of HeLa cells using Caspase 9 Polyclonal Antibody (Product # PA5-19904) at 5 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.