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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- NB500-209 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-209, RRID:AB_10001948
- Product name
- Mouse Monoclonal Caspase-9 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.2 mg
- Concentration
- 1.0 mg/ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The induction of apoptosis and autophagy by Wasabia japonica extract in colon cancer.
Expression of SIRT1 and apoptosis-related proteins is predictive for lymph node metastasis and disease-free survival in luminal A breast cancer.
Apocytochrome c blocks caspase-9 activation and Bax-induced apoptosis.
Hsuan SW, Chyau CC, Hung HY, Chen JH, Chou FP
European journal of nutrition 2016 Mar;55(2):491-503
European journal of nutrition 2016 Mar;55(2):491-503
Expression of SIRT1 and apoptosis-related proteins is predictive for lymph node metastasis and disease-free survival in luminal A breast cancer.
Kim H, Lee KH, Park IA, Chung YR, Im SA, Noh DY, Han W, Moon HG, Jung YY, Ryu HS
Virchows Archiv : an international journal of pathology 2015 Nov;467(5):563-70
Virchows Archiv : an international journal of pathology 2015 Nov;467(5):563-70
Apocytochrome c blocks caspase-9 activation and Bax-induced apoptosis.
Martin AG, Fearnhead HO
The Journal of biological chemistry 2002 Dec 27;277(52):50834-41
The Journal of biological chemistry 2002 Dec 27;277(52):50834-41
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - The pro and active forms of Caspase 9 detected in active 293 cell lysate (lane 2). Lane 1: inactive 293 cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - Western blot analysis of Jurkat (A) and HeLa (B) cell lysate using Caspase 9 (NB500-209) antibody at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Caspase-9 Antibody (LAP6 96-2-22) [NB500-209] - Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Staurosporine (STS) for 3 hours, loaded at 0.2 mg/mL. A specific band was detected for Caspase 9 at approximately 53 kDa (as indicated) using 20 ug/mL of Mouse Anti-Caspase 9 Monoclonal Antibody (Catalog # NB500-209). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - IHC analysis of paraffin-embedded human breast carcinomausing Caspase 9 antibody at 1:25. Image from verified customer review.
