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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
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- Product number
- PA1595 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Caspase 9/CASP9 Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for CASPASE 9/CASP9 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CASPASE 9/CASP9 information: Molecular Weight: 46281 MW; Tissue Specificity: Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Sdy-1 Executes Antitumor Activity in HepG2 and HeLa Cancer Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway.
Thiazolidinedione-Based Structure Modification of Celastrol Provides Thiazolidinedione-Conjugated Derivatives as Potent Agents against Non-Small-Cell Lung Cancer Cells through a Mitochondria-Mediated Apoptotic Pathway.
PPM-18, an Analog of Vitamin K, Induces Autophagy and Apoptosis in Bladder Cancer Cells Through ROS and AMPK Signaling Pathways.
Loading Auristatin PE onto boron nitride nanotubes and their effects on the apoptosis of Hep G2 cells.
Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs.
Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells.
Sun M, Zhou D, Wu J, Zhou J, Xu J
Marine drugs 2022 Feb 5;20(2)
Marine drugs 2022 Feb 5;20(2)
Thiazolidinedione-Based Structure Modification of Celastrol Provides Thiazolidinedione-Conjugated Derivatives as Potent Agents against Non-Small-Cell Lung Cancer Cells through a Mitochondria-Mediated Apoptotic Pathway.
Fu X, Mao Q, Zhang B, Lv J, Ping K, Zhang P, Lin F, Zhao J, Feng Y, Yang J, Wang H, Zhang L, Mou Y, Wang S
Journal of natural products 2022 Apr 22;85(4):1147-1156
Journal of natural products 2022 Apr 22;85(4):1147-1156
PPM-18, an Analog of Vitamin K, Induces Autophagy and Apoptosis in Bladder Cancer Cells Through ROS and AMPK Signaling Pathways.
Lu H, Mei C, Yang L, Zheng J, Tong J, Duan F, Liang H, Hong L
Frontiers in pharmacology 2021;12:684915
Frontiers in pharmacology 2021;12:684915
Loading Auristatin PE onto boron nitride nanotubes and their effects on the apoptosis of Hep G2 cells.
Li W, Xie X, Wu T, Lin H, Luo L, Yang H, Li J, Xin Y, Lin X, Chen Y
Colloids and surfaces. B, Biointerfaces 2019 Sep 1;181:305-314
Colloids and surfaces. B, Biointerfaces 2019 Sep 1;181:305-314
Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs.
Guan YQ, Li Z, Chen J, Tao H, Wang W, Zheng Z, Li L, Liu JM
Biomaterials 2011 May;32(14):3637-46
Biomaterials 2011 May;32(14):3637-46
Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells.
Wu YF, Cao MF, Gao YP, Chen F, Wang T, Zumbika EP, Qian KX
World journal of gastroenterology 2004 Oct 15;10(20):2944-8
World journal of gastroenterology 2004 Oct 15;10(20):2944-8
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Anti-Caspase-9 antibody, PA1595, Western blottingAll lanes: Anti Caspase-9 (PA1595) at 0.5ug/mlLane 1: SMMC Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugLane 3: CEM Whole Cell Lysate at 40ugLane 4: JURKAT Whole Cell Lysate at 40ugLane 5: RAJI Whole Cell Lysate at 40ugLane 6: HELA Whole Cell Lysate at 40ugPredicted bind size: 35KDObserved bind size: 35KD
- Additional image
