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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP21661SU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP21661SU-S, RRID:AB_10757216
- Product name
- anti Caspase-9
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length human Caspase-9 protein (pro-form) was used as immunogen (Knoblach et al, 2002).
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Vial size
- 50 µl
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase-9. Various tissue lysates were prepared from human autospsy material and normalized for total protein. Most tissues contained the ~50 kDa pro-Caspase-9 protein. Cleaved Caspase-9 was identified in brain cortex and peripheral nerve tissue samples. Pro-C9: recombinant human pro-Caspase-9 (full-length) protein. PRO: pro-Caspase-9. L: large subunit of cleaved Caspase-9. S: small subunit of cleaved Caspase-9.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Caspase-9 expression in formalin-fixed, paraffin-embedded mouse brain tissue sections using AP21661SU-S at 1/2000. Hematoxylin-eosin counterstain. A: Brain striatum. B: Brain stem motor neuron.
