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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [9]
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- Product number
- LS-C114534 - Provider product page
- Provider
- LSBio
- Product name
- BRAF / B-Raf Antibody (clone OTI5A9) LS-C114534
- Antibody type
- Monoclonal
- Description
- Protein A/G purified
- Reactivity
- Human, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI5A9
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of extracts (35 ug) from 9 different cell lines by using anti-BRAF monoclonal antibody.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BRAF mouse monoclonal antibody.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-BRAF mouse monoclonal antibody.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Human lung tissue using anti-BRAF mouse monoclonal antibody.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Human pancreas tissue using anti-BRAF mouse monoclonal antibody.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Human endometrium tissue using anti-BRAF mouse monoclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-BRAF mouse monoclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC of paraffin-embedded Human lung tissue using anti-BRAF mouse monoclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC of paraffin-embedded Human pancreas tissue using anti-BRAF mouse monoclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC of paraffin-embedded Human endometrium tissue using anti-BRAF mouse monoclonal antibody.
