TA321644

antibody from OriGene

Targeting: BRAF BRAF1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA321644

Provider

OriGene

Product name

Anti-BRAF Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to a region derived from 591-607 amino acids of Human v-raf murine sarcoma viral oncogene homolog B1

Description

Anti-BRAF Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Concentration

2.1mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 84 kDa . Positive control: Hela and HT-29 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Hela cell lysate Lane2: HT-29 cell lysate Lysates: 50 ug per lane Primary antibody: 1/1400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm. Positive control: Human tonsil tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human tonsil tissue using BRAF antibody at dilution 1/60, on the right is treated with the synthetic peptide. (Original magnification:x200)

Validation comment

IHC