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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [7]
- Flow cytometry [1]
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- Product number
- GTX84806 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84806, RRID:AB_10729741
- Product name
- B-Raf antibody [1E2]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
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- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BRAF monoclonal antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-BRAF mouse monoclonal antibody (GTX84806) immunofluorescent staining of COS7 cells transiently transfected with BRAF
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-BRAF mouse monoclonal antibody (GTX84806).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-BRAFmouse monoclonal antibody. (GTX84806, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-BRAFmouse monoclonal antibody. (GTX84806, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue using anti-BRAFmouse monoclonal antibody. (GTX84806, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-BRAFmouse monoclonal antibody. (GTX84806, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BRAF mouse monoclonal antibody. (GTX84806, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-BRAFmouse monoclonal antibody. (GTX84806, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BRAFmouse monoclonal antibody. (GTX84806, Dilution 1:50)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY BRAF
