Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX84819 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84819, RRID:AB_10731066
- Product name
- B-Raf antibody [5A9]
- Antibody type
- Monoclonal
- Reactivity
- Human, Simian
- Host
- Mouse
- Storage
- For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BRAF monoclonal antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-BRAF mouse monoclonal antibody (GTX84819) immunofluorescent staining of COS7 cells transiently transfected with BRAF
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BRAF mouse monoclonal antibody. (GTX84819)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-BRAF mouse monoclonal antibody. (GTX84819)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-BRAF mouse monoclonal antibody. (GTX84819)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-BRAF mouse monoclonal antibody. (GTX84819)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-BRAF mouse monoclonal antibody. (GTX84819)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either BRAF overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BRAF antibody (GTX84819), and then analyzed by flow cytometry.