PAB29515

antibody from Abnova Corporation

Targeting: BRAF BRAF1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29515

Provider

Abnova Corporation

Product name

BRAF polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human BRAF.

Antigen sequence

PIPQEEASLAETALTSGSSPSAPASDSIGPQILTS
PSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPN
VHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPP
ASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRM
KTLGRRDSS


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with BRAF polyclonal antibody (Cat # PAB29515).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with BRAF polyclonal antibody (Cat # PAB29515) shows moderate cytoplasmic positivity in cells in seminiferus ducts at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)