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- Western blot [1]
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- Product number
- GTX33595 - Provider product page
- Provider
- GeneTex
- Product name
- B-Raf (V600E Mutant Specific) antibody [RM8]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Nanoswitch-linked immunosorbent assay (NLISA) for fast, sensitive, and specific protein detection.
Hansen CH, Yang D, Koussa MA, Wong WP
Proceedings of the National Academy of Sciences of the United States of America 2017 Sep 26;114(39):10367-10372
Proceedings of the National Academy of Sciences of the United States of America 2017 Sep 26;114(39):10367-10372
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western Blot analysis of cell lysates prepared from cell lines expressing endogenous mutant (V600E) BRAF or wild type (WT) protein.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sandwich ELISA performed on the lysates of 293T cells overexpressing BRAF V600E or BRAF wild type His-tag proteins, shows the specificity of RM8 to the V600E mutation. His-tag fusion proteins were captured by an Anti-His-Tag antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of formalin fixed and paraffin embedded melanoma tissue sections.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of formalin fixed and paraffin embedded human cell line sections. WiDr Cells (400X, Positive)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of formalin fixed and paraffin embedded human cell line sections. SW40 Cells (400X, Negative)
