PAB0421

antibody from Abnova Corporation

Targeting: BRAF BRAF1

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB0421

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB0421, RRID:AB_982370

Product name

BRAF (phospho S602) polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S602 of human BRAF.

Reactivity

Human

Host

Rabbit

Vial size

400 μl

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Calcium restriction allows cAMP activation of the B-Raf/ERK pathway, switching cells to a cAMP-dependent growth-stimulated phenotype.

Yamaguchi T, Wallace DP, Magenheimer BS, Hempson SJ, Grantham JJ, Calvet JP
The Journal of biological chemistry 2004 Sep 24;279(39):40419-30

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Alternative mutations of BRAF, RET and NTRK1 are associated with similar but distinct gene expression patterns in papillary thyroid cancer.

Frattini M, Ferrario C, Bressan P, Balestra D, De Cecco L, Mondellini P, Bongarzone I, Collini P, Gariboldi M, Pilotti S, Pierotti MA, Greco A
Oncogene 2004 Sep 23;23(44):7436-40

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BRAF kinase gene V599E mutation in growing melanocytic lesions.

Loewe R, Kittler H, Fischer G, Faé I, Wolff K, Petzelbauer P
The Journal of investigative dermatology 2004 Oct;123(4):733-6

Other assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Dot blot analysis of BRAF (phospho S601) polyclonal antibody (Cat # PAB0421) and BRAF Non Phospho-specific Pab on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.

Validation comment

Dot Blot (Peptide)