PAB0422

antibody from Abnova Corporation

Targeting: BRAF BRAF1

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB0422

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB0422, RRID:AB_982371

Product name

BRAF (phospho T439) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of BRAF.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Calcium restriction allows cAMP activation of the B-Raf/ERK pathway, switching cells to a cAMP-dependent growth-stimulated phenotype.

Yamaguchi T, Wallace DP, Magenheimer BS, Hempson SJ, Grantham JJ, Calvet JP
The Journal of biological chemistry 2004 Sep 24;279(39):40419-30

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Alternative mutations of BRAF, RET and NTRK1 are associated with similar but distinct gene expression patterns in papillary thyroid cancer.

Frattini M, Ferrario C, Bressan P, Balestra D, De Cecco L, Mondellini P, Bongarzone I, Collini P, Gariboldi M, Pilotti S, Pierotti MA, Greco A
Oncogene 2004 Sep 23;23(44):7436-40

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BRAF kinase gene V599E mutation in growing melanocytic lesions.

Loewe R, Kittler H, Fischer G, Faé I, Wolff K, Petzelbauer P
The Journal of investigative dermatology 2004 Oct;123(4):733-6

Other assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Dot blot analysis of BRAF (phospho T439) polyclonal antibody (Cat # PAB0422) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5 ug/mL. P-Pab : phospho-antibody; P-Peptide : phospho-peptide; NP-Peptide : non-phospho-peptide.

Validation comment

Dot Blot (Peptide)