Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB3946 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3946, RRID:AB_1571764
- Product name
- BRAF polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of BRAF.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Histologic and immunohistochemical predictors of clinical behavior for feline diffuse iris melanoma.
Suppression of BRAF(V599E) in human melanoma abrogates transformation.
BRAF mutations in non-Hodgkin's lymphoma.
Mutations of the BRAF gene in human cancer.
Wiggans KT, Reilly CM, Kass PH, Maggs DJ
Veterinary ophthalmology 2016 Jan 24;
Veterinary ophthalmology 2016 Jan 24;
Suppression of BRAF(V599E) in human melanoma abrogates transformation.
Hingorani SR, Jacobetz MA, Robertson GP, Herlyn M, Tuveson DA
Cancer research 2003 Sep 1;63(17):5198-202
Cancer research 2003 Sep 1;63(17):5198-202
BRAF mutations in non-Hodgkin's lymphoma.
Lee JW, Yoo NJ, Soung YH, Kim HS, Park WS, Kim SY, Lee JH, Park JY, Cho YG, Kim CJ, Ko YH, Kim SH, Nam SW, Lee JY, Lee SH
British journal of cancer 2003 Nov 17;89(10):1958-60
British journal of cancer 2003 Nov 17;89(10):1958-60
Mutations of the BRAF gene in human cancer.
Davies H, Bignell GR, Cox C, Stephens P, Edkins S, Clegg S, Teague J, Woffendin H, Garnett MJ, Bottomley W, Davis N, Dicks E, Ewing R, Floyd Y, Gray K, Hall S, Hawes R, Hughes J, Kosmidou V, Menzies A, Mould C, Parker A, Stevens C, Watt S, Hooper S, Wilson R, Jayatilake H, Gusterson BA, Cooper C, Shipley J, Hargrave D, Pritchard-Jones K, Maitland N, Chenevix-Trench G, Riggins GJ, Bigner DD, Palmieri G, Cossu A, Flanagan A, Nicholson A, Ho JW, Leung SY, Yuen ST, Weber BL, Seigler HF, Darrow TL, Paterson H, Marais R, Marshall CJ, Wooster R, Stratton MR, Futreal PA
Nature 2002 Jun 27;417(6892):949-54
Nature 2002 Jun 27;417(6892):949-54
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of BRAF (arrow) using rabbit BRAF polyclonal antibody (Cat # PAB3946). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with BRAF polyclonal antibody (Cat # PAB3946) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)