PAB7907

antibody from Abnova Corporation

Targeting: BRAF BRAF1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB7907

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB7907, RRID:AB_1676141

Product name

BRAF (phospho S446) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of BRAF .

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Rheb inhibits C-raf activity and B-raf/C-raf heterodimerization.

Karbowniczek M, Robertson GP, Henske EP
The Journal of biological chemistry 2006 Sep 1;281(35):25447-56

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Functional analysis of the regulatory requirements of B-Raf and the B-Raf(V600E) oncoprotein.

Brummer T, Martin P, Herzog S, Misawa Y, Daly RJ, Reth M
Oncogene 2006 Oct 12;25(47):6262-76

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BAY 43-9006 exhibits broad spectrum oral antitumor activity and targets the RAF/MEK/ERK pathway and receptor tyrosine kinases involved in tumor progression and angiogenesis.

Wilhelm SM, Carter C, Tang L, Wilkie D, McNabola A, Rong H, Chen C, Zhang X, Vincent P, McHugh M, Cao Y, Shujath J, Gawlak S, Eveleigh D, Rowley B, Liu L, Adnane L, Lynch M, Auclair D, Taylor I, Gedrich R, Voznesensky A, Riedl B, Post LE, Bollag G, Trail PA
Cancer research 2004 Oct 1;64(19):7099-109

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot of human Jurkat cells treated with calyculin A(100 nM) for 30 min. The blots were untreated (lane 1) or treated (lane 2) with lambda phosphatase and probed with BRAF (phospho S446) polyclonal antibody (Cat # PAB7907).