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Other assayAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Other assay [1]
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- Product number
- PAB8096 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB8096, RRID:AB_1676140
- Product name
- BRAF (phospho S445) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of BRAF.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Calcium restriction allows cAMP activation of the B-Raf/ERK pathway, switching cells to a cAMP-dependent growth-stimulated phenotype.
Alternative mutations of BRAF, RET and NTRK1 are associated with similar but distinct gene expression patterns in papillary thyroid cancer.
BRAF kinase gene V599E mutation in growing melanocytic lesions.
Yamaguchi T, Wallace DP, Magenheimer BS, Hempson SJ, Grantham JJ, Calvet JP
The Journal of biological chemistry 2004 Sep 24;279(39):40419-30
The Journal of biological chemistry 2004 Sep 24;279(39):40419-30
Alternative mutations of BRAF, RET and NTRK1 are associated with similar but distinct gene expression patterns in papillary thyroid cancer.
Frattini M, Ferrario C, Bressan P, Balestra D, De Cecco L, Mondellini P, Bongarzone I, Collini P, Gariboldi M, Pilotti S, Pierotti MA, Greco A
Oncogene 2004 Sep 23;23(44):7436-40
Oncogene 2004 Sep 23;23(44):7436-40
BRAF kinase gene V599E mutation in growing melanocytic lesions.
Loewe R, Kittler H, Fischer G, Faé I, Wolff K, Petzelbauer P
The Journal of investigative dermatology 2004 Oct;123(4):733-6
The Journal of investigative dermatology 2004 Oct;123(4):733-6
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Supportive validation
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- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot blot analysis of BRAF (phospho S445) polyclonal antibody (Cat # PAB8096) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
- Validation comment
- Dot Blot (Peptide)
