Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB10422 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10422, RRID:AB_11183575
- Product name
- BRAF monoclonal antibody, clone 1H12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant BRAF.
- Isotype
- IgG
- Antibody clone number
- 1H12
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of BRAF monoclonal antibody, clone 1H12 (Cat # MAB10422) against HeLa (1), HL-60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis of MCF-7 (A) and HepG2 (B) cells using BRAF monoclonal antibody, clone 1H12 (Cat # MAB10422) (green).Red: Actin filaments have been labeled with DY-554 phalloidin.Blue: DRAQ5 fluorescent DNA dye.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human testis tissue using BRAF monoclonal antibody, clone 1H12 (Cat # MAB10422).
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)