TA500446

antibody from OriGene

Targeting: BRAF BRAF1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500446

Provider

OriGene

Proper citation

OriGene Cat#TA500446, RRID:AB_2054740

Product name

Anti-BRAF mouse monoclonal antibody, clone OTI3B12 (formerly 3B12)

Antibody type

Monoclonal

Description

Anti-BRAF mouse monoclonal antibody, clone OTI3B12 (formerly 3B12)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

BRAF

Isotype

IgG

Antibody clone number

OTI3B12

Vial size

100 µl

Concentration

0.97 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (RC211013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-BRAFmonoclonal antibody.

Validation comment

WB

Immunoprecipitation

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunoprecipitation(IP) of BRAF by using TrueMab monoclonal anti-BRAF antibodies (Negative control: IP without adding anti-BRAF antibody.). For each experiment, 500ul of DDK tagged BRAF overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-BRAF antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Validation comment

IP