Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [6]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP1-47668 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-47668, RRID:AB_10010625
- Product name
- Mouse Monoclonal B-Raf Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified. This antibody is specific for Homo sapiens v-raf murine sarcoma viral oncogene homolog B1 (BRAF).
- Reactivity
- Human, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references An epitranscriptomic mechanism underlies selective mRNA translation remodelling in melanoma persister cells.
Shen S, Faouzi S, Bastide A, Martineau S, Malka-Mahieu H, Fu Y, Sun X, Mateus C, Routier E, Roy S, Desaubry L, André F, Eggermont A, David A, Scoazec JY, Vagner S, Robert C
Nature communications 2019 Dec 16;10(1):5713
Nature communications 2019 Dec 16;10(1):5713
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: B-Raf Antibody (OTI5A9) [NBP1-47668] - Analysis of extracts (35ug) from 9 different cell lines by using anti-B Raf monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: B-Raf Antibody (OTI5A9) [NBP1-47668] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antibody
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: B-Raf Antibody (OTI5A9) [NBP1-47668] - The images depict immunostaining of representative PTC cases harboring BRAF wild-type (A, B) and BRAF V600E mutation (C, D) proven by NGS; varying degrees of immunoreactivity for BRAF is shown both in the intranuclear inclusions (arrows) and in the cytoplasm. Original magnifications: 1,000 X.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-B Raf mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-B Raf mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Human endometrium tissue using anti-B Raf mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Human lung tissue using anti-B Raf mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Human pancreas tissue using anti-B Raf mouse monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: B-Raf Antibody (OTI5A9) [NBP1-47668] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-B Raf antibody, and then analyzed by flow cytometry.