Ab091608

antibody from Aladdin Scientific

Targeting: BRAF BRAF1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab091608

Provider

Aladdin Scientific

Product name

BRAF Mouse mAb

Antibody type

Monoclonal

Description

Mouse anti Human BRAF Antibody, Monoclonal (125CT13.3.5), could be used for WB, IF, ICC and so on.Application:WB: 1/500-1/2000ICC/IF: 1/10-1/50Protein FunctionInvolved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

AA 4-365


Antibody clone number

125CT13.3.5

Vial size

100_l,10_l,1ml,50_l

Concentration

0,5 mg/ml

Storage

Store at 4¡C short term (2 weeks). Store at -20¡C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

BRAF Mouse mAb (Ab091608) - Western Blot All lanes: BRAF Mouse mAb (Ab091608) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 86 kDa Observed band size: 85 kDa Exposure time: 17.9 s

Immunocytochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

BRAF Mouse mAb (Ab091608) - ICC/IF Fluorescent image of C2C12 cells stained with BRAF Antibody (Ab091608). C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody 1:25, 1 h at 37℃. For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used 1:400, 50 min at 37℃. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin 7 units/mL, 1 h at 37℃. BRAF immunoreactivity is localized to Cytoplasm significantly.