Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP1-89942 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89942, RRID:AB_11031861
- Product name
- Rabbit Polyclonal B-Raf Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human B-Raf antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Tuneable endogenous mammalian target complementation via multiplexed plasmid-based recombineering.
Beltran-Sastre V, Benisty H, Burnier J, Berger I, Serrano L, Kiel C
Scientific reports 2015 Nov 27;5:17432
Scientific reports 2015 Nov 27;5:17432
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: B-Raf Antibody [NBP1-89942] - Simple Western lane view shows a specific band for BRAF in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: B-Raf Antibody [NBP1-89942] - Electropherogram image(s) of corresponding Simple Western lane view. B-Raf antibody was used at 1:20 dilution on RT-4 and U-251MG sp lysates(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: B-Raf Antibody [NBP1-89942] - Analysis in human cell line MOLT-4.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: B-Raf Antibody [NBP1-89942] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human colorectal cancer shows moderate cytoplasmic positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human kidney shows moderate cytoplasmic positivity in distal tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human liver shows weak cytoplasmic positivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.