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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-14933 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- B-Raf Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with chicken and mouse based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.34 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of B-RAF in MCF-7 lysates (35 µg) using a B-RAF polyclonal antibody (Product # PA5-14933) at a dilution of 1:500 followed by detection using an HRP-conjugated goat anti-rabbit IgG (H+L) secondary antibody at a dilution of 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain tissue using a B-RAF polyclonal antibody (Product # PA5-14933), followed by HRP-conjugated secondary antibody and DAB staining.
