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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB3424 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat B-Raf Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat B-Raf . No cross-reactivity with recombinant human Raf-1 is observed. Cross-reactivity with A-Raf has not been tested.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P15056- Isotype
- IgG
- Antibody clone number
- 336016
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat B-Raf by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat B-Raf Monoclonal Antibody (Catalog # MAB3424) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Active Raf-1 (Y340E Y341E), aa 306-648 (Catalog # 4540-KS) and recombinant human B-Raf (5 ng/lane) were included. A specific band for B-Raf was detected at approximately 95 kDa (as indicated). An unidentified band at 55 kDa was also detected. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
