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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00001634-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00001634-M01, RRID:AB_425394
- Product name
- DCN monoclonal antibody (M01), clone 3H4-1F4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant DCN.
- Antigen sequence
MKATIILLLLAQVSWAGPFQQRGLFDFMLEDEASG
IGPEVPDDRDFEPSLGPVCPFRCQCHLRVVQCSDL
GLDKVPKDLPPDTTLLDLQNNKITEIKDGDFKNLK
NLHALILVNNKISKVSPGAFTPLVKLERLYLSKNQ
LKELPEKMPKTLQELRAHENEITKVRKVTFNGLNQ
MIVIELGTNPLKSSGIENGAFQGMKKLSYIRIADT
NITSIPQGLPPSLTELHLDGNKISRVDAASLKGLN
NLAKLGLSFNSISAVDNGSLANTPHLRELHLDNNK
LTRVPGGLAEHKYIQVVYLHNNNISVVGSSDFCPP
GHNTKKASYSGVSLFSNPVQYWEIQPSTFRCVYVR
SAIQLGNYK- Isotype
- IgG
- Antibody clone number
- 3H4-1F4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Smoking is associated with lower amounts of arterial type I collagen and decorin.
Faarvang AS, Rørdam Preil SA, Nielsen PS, Beck HC, Kristensen LP, Rasmussen LM
Atherosclerosis 2016 Apr;247:201-6
Atherosclerosis 2016 Apr;247:201-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of DCN expression in transfected 293T cell line by DCN monoclonal antibody (M01), clone 3H4-1F4.Lane 1: DCN transfected lysate (Predicted MW: 39.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged DCN is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to DCN on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
