Antibody data
- Antibody Data
 - Antigen structure
 - References [0]
 - Comments [0]
 - Validations
 - Western blot [2]
 - Immunohistochemistry [1]
 - Flow cytometry [1]
 
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Validation data
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- Product number
 - F54863 - Provider product page

 - Provider
 - NSJ Bioreagents
 - Product name
 - DCN Antibody / Decorin
 - Antibody type
 - Polyclonal
 - Description
 - This highly specific DCN antibody is suitable for use in Immunohistochemistry/Flow cytometry/Western blot applications with human and mouse samples.
 - Reactivity
 - Human, Mouse
 - Host
 - Rabbit
 - Conjugate
 - Unconjugated
 - Vial size
 - 0.08 ml, 0.4 ml
 - Concentration
 - In 1X PBS, pH 7.4, with 0.09% sodium azide
 - Storage
 - Aliquot the DCN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
 
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					Supportive validation
					
									
				
				- Submitted by
 - NSJ Bioreagents (provider)
 - Main image
 
- Experimental details
 - Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with DCN antibody.
 
- Submitted by
 - NSJ Bioreagents (provider)
 - Main image
 
- Experimental details
 - Western blot testing of mouse heart tissue lysate with DCN antibody. Predicted molecular weight ~40 kDa.
 
							
					Supportive validation
					
									
				
				- Submitted by
 - NSJ Bioreagents (provider)
 - Main image
 
- Experimental details
 - IHC testing of FFPE human hepatocellular carcinoma tissue with DCN antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
 
							
					Supportive validation
					
									
				
		- Submitted by
 - NSJ Bioreagents (provider)
 - Main image
 
- Experimental details
 - Flow cytometry testing of human HepG2 cells with DCN antibody; Blue=isotype control, Green= DCN antibody.