NB100-65301
antibody from Novus Biologicals
Targeting: SIGLEC1
CD169, dJ1009E24.1, FLJ00051, FLJ00055, FLJ00073, FLJ32150, sialoadhesin, SIGLEC-1, SN
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-65301 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-65301, RRID:AB_2254743
- Product name
- Mouse Monoclonal Siglec-1/CD169 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. NB100-65301 recognizes the rat CD169 cell surface antigen, a 185kD molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.
- Reactivity
- Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (ED3) [NB100-65301] - Rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent.