12-1699-41
antibody from Invitrogen Antibodies
Targeting: SIGLEC1
CD169, dJ1009E24.1, FLJ00051, FLJ00055, FLJ00073, FLJ32150, sialoadhesin, SIGLEC-1, SN
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 12-1699-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD169 (Siglec-1) Monoclonal Antibody (7-239), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 7-239 monoclonal antibody reacts with human CD169, also known as Siglec-1 and Sialoadhesin. This type I transmembrane protein is a member of the sialic acid binding Ig-like lectin (Siglec) family. Expressed on macrophages and dendritic cells in peripheral blood, lymph nodes, and spleen in humans, CD169 binds glycoproteins and glycolipids containing N-acetylneuraminyl alpha 2-3-galactose. CD169 expression can also be induced on CD14+ monocytes by interferon-alpha and interferon-gamma to mediate HIV-1 infection. Finally, CD169 has also been shown to be involved in antigen presentation to invariant NK T cells. This monoclonal antibody has been reported to inhibit erythrocyte binding to CD169-expressing cells. Applications Reported: This 7-239 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 7-239 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- 7-239
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Decreased expression of a phagocytic receptor Siglec-1 on alveolar macrophages in chronic obstructive pulmonary disease.
Phenotypic and Functional Characterization of Monoclonal Antibodies with Specificity for Rhesus Macaque CD200, CD200R and Mincle.
Tanno A, Fujino N, Yamada M, Sugiura H, Hirano T, Tanaka R, Sano H, Suzuki S, Okada Y, Ichinose M
Respiratory research 2020 Jan 28;21(1):30
Respiratory research 2020 Jan 28;21(1):30
Phenotypic and Functional Characterization of Monoclonal Antibodies with Specificity for Rhesus Macaque CD200, CD200R and Mincle.
Byrareddy SN, Little D, Mayne AE, Villinger F, Ansari AA
PloS one 2015;10(10):e0140689
PloS one 2015;10(10):e0140689
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells, cultured for 3 days, with Anti-Human CD14 APC (Product # 17-0149-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD169 (Siglec-1) PE (right). Cells in the monocyte gate were used for analysis.
- Conjugate
- Yellow dye