12-1699-42
antibody from Invitrogen Antibodies
Targeting: SIGLEC1
CD169, dJ1009E24.1, FLJ00051, FLJ00055, FLJ00073, FLJ32150, sialoadhesin, SIGLEC-1, SN
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [4]
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Validation data
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- Product number
- 12-1699-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD169 (Siglec-1) Monoclonal Antibody (7-239), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 7-239 monoclonal antibody reacts with human CD169, also known as Siglec-1 and Sialoadhesin. This type I transmembrane protein is a member of the sialic acid binding Ig-like lectin (Siglec) family. Expressed on macrophages and dendritic cells in peripheral blood, lymph nodes, and spleen in humans, CD169 binds glycoproteins and glycolipids containing N-acetylneuraminyl alpha 2-3-galactose. CD169 expression can also be induced on CD14+ monocytes by interferon-alpha and interferon-gamma to mediate HIV-1 infection. Finally, CD169 has also been shown to be involved in antigen presentation to invariant NK T cells. This monoclonal antibody has been reported to inhibit erythrocyte binding to CD169-expressing cells. Applications Reported: This 7-239 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 7-239 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- 7-239
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Decreased expression of a phagocytic receptor Siglec-1 on alveolar macrophages in chronic obstructive pulmonary disease.
Phenotypic and Functional Characterization of Monoclonal Antibodies with Specificity for Rhesus Macaque CD200, CD200R and Mincle.
Tanno A, Fujino N, Yamada M, Sugiura H, Hirano T, Tanaka R, Sano H, Suzuki S, Okada Y, Ichinose M
Respiratory research 2020 Jan 28;21(1):30
Respiratory research 2020 Jan 28;21(1):30
Phenotypic and Functional Characterization of Monoclonal Antibodies with Specificity for Rhesus Macaque CD200, CD200R and Mincle.
Byrareddy SN, Little D, Mayne AE, Villinger F, Ansari AA
PloS one 2015;10(10):e0140689
PloS one 2015;10(10):e0140689
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells, cultured for 3 days, with Anti-Human CD14 APC (Product # 17-0149-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD169 (Siglec-1) PE (right). Cells in the monocyte gate were used for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 2 Representative flow cytometric profiles of CD200, CD200R, Mincle, Siglec-1 and Siglec-3 expression by the U937 (panel a) and THP-1 (panel b) cell lines and by the gated population of CD14+ cells from PBMCs from a representative normal rhesus macaque (panel c) and for comparison the profile of the gated population of CD14+ cells from the human PBMCs stained with our panel of anti-CD200, CD200R and Mincle antibodies (panel d) and the commercially obtained monoclonal antibodies with specificity for human CD200, CD200R and Mincle (panel e). The profile in red reflects the use of anti-mouse Ig (control) and the one in blue reflects the profile noted with the appropriate anti-lectin monoclonal antibody (mAb). The numbers in red and blue are the MFI values noted for the anti-mouse Ig control and the anti-lectin mAb, respectively.
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 3 Representative flow cytometric profile of CD200, CD200R, Mincle, Siglec-1 and Siglec-3 expression on the gated population of CD14 + cells isolated from the bone marrow (panel a), spleen (panel b), liver (panel c) and mononuclear cells isolated from rectal biopsy samples (panel d) from a representative healthy normal rhesus macaque. The profile in red reflects the use of anti-mouse Ig (control) and the one in blue reflects the profile noted with the appropriate anti-lectin monoclonal antibody (mAb). The numbers in red and blue are the MFI values noted for the anti-mouse Ig control and the anti-lectin mAb, respectively.
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Expression levels of phagocytic receptors on human alveolar macrophages. a FACS gating strategy to delineate alveolar macrophages. Cell suspension was collected by perfusing and lavaging human lung tissues with saline. Alveolar macrophages were detected as live, FSC hi /SSC hi , single cell-gated CD45 + /CD206 + cells in the cell suspension. b Representative histograms for the expression levels of FcgammaRI, CD11b, macrophage scavenger receptor-1 (MSR-1), CD36 and Siglec-1, on FSC hi /SSC hi /CD45 + /CD206 + alveolar macrophages of control never-smokers (CNS, n = 11), control ex-smokers (CES, n = 9) and COPD ex-smokers (COPD, n = 11). Specific staining, open; isotype control, shaded
- Conjugate
- Yellow dye