62-1699-42
antibody from Invitrogen Antibodies
Targeting: SIGLEC1
CD169, dJ1009E24.1, FLJ00051, FLJ00055, FLJ00073, FLJ32150, sialoadhesin, SIGLEC-1, SN
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- 62-1699-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD169 (Siglec-1) Monoclonal Antibody (7-239), Super Bright™ 436, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 7-239 monoclonal antibody reacts with human CD169, also known as Siglec-1 and Sialoadhesin. This type I transmembrane protein is a member of the sialic acid binding Ig-like lectin (Siglec) family. Expressed on macrophages and dendritic cells in peripheral blood, lymph nodes, and spleen in humans, CD169 binds glycoproteins and glycolipids containing N-acetylneuraminyl alpha 2-3-galactose. CD169 expression can also be induced on CD14+ monocytes by interferon-alpha and interferon-gamma to mediate HIV-1 infection. Finally, CD169 has also been shown to be involved in antigen presentation to invariant NK T cells. This monoclonal antibody has been reported to inhibit erythrocyte binding to CD169-expressing cells. Applications Reported: This 7-239 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 7-239 antibody has been pre-diluted and tested by flow cytometric analysis. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7-239
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were cultured for 3 days. Cells were then stained with CD14 Monoclonal Antibody, PE (Product # 12-0149) and Mouse IgG1 kappa Isotype Control, Super Bright 436 (Product # 62-4714) (left) or CD169 (Siglec-1) Monoclonal Antibody, Super Bright 436 (right). Cells in the monocyte gate were used for analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were cultured for 3 days. Cells were then stained with CD14 Monoclonal Antibody, PE (Product # 12-0149-42) and Mouse IgG1 kappa Isotype Control, Super Bright 436 (Product # 62-4714-82) (left) or CD169 (Siglec-1) Monoclonal Antibody, Super Bright 436 (right). Cells in the monocyte gate were used for analysis.