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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-110082 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCMT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KSGGASHSEL IHNLRKNGII KTDKVFEVML ATDRSHYAKC NPYMDSPQSI GFQATISAPH MHAYALELLF DQLHEGAKAL DVGSGSGILT ACFARMVGCT GKVIGIDHIK ELVDDSVNNV RKDDPTLLSS GRVQLVVGDG RMGYAEEAPY DAIHVGAAAP VVPQALIDQL KPGGRLILPV GPAGGNQMLE QYDKLQDGSI KMKPLMGVIY VPLTDKEKQW SRDEL
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.11 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of PCMT1 in HeLa cells. Samples were incubated in polyclonal PCMT1 antibody (Product # PA5-110082) using a dilution of 1:100, followed by DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PCMT1 in HeLa cells. Samples were incubated with PCMT1 Polyclonal antibody (Product # PA5-110082). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of PCMT1 in 200 μg extracts of HepG2 cells. Samples were precipitated with 1 μg PCMT1 Polyclonal antibody (Product # PA5-110082). Western blot was performed from the immunoprecipitate using PCMT1 Polyclonal antibody (Product # PA5-110082) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of PCMT1 in HePG2 cells. Samples were incubated in polyclonal PCMT1 antibody (Product # PA5-110082) using a dilution of 1:1000.
