Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [6]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-85806 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85806, RRID:AB_11035901
- Product name
- Rabbit Polyclonal PCMT1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human PCMT1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Proteome-wide epitope mapping of antibodies using ultra-dense peptide arrays.
Forsström B, Axnäs BB, Stengele KP, Bühler J, Albert TJ, Richmond TA, Hu FJ, Nilsson P, Hudson EP, Rockberg J, Uhlen M
Molecular & cellular proteomics : MCP 2014 Jun;13(6):1585-97
Molecular & cellular proteomics : MCP 2014 Jun;13(6):1585-97
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCMT1 Antibody [NBP1-85806] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells). Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCMT1 Antibody [NBP1-85806] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human Cerebral Cortex tissue
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCMT1 Antibody [NBP1-85806] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCMT1 Antibody [NBP1-85806] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18 Lane 2: Human cell line RT-4
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCMT1 Antibody [NBP1-85806] - Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCMT1 Antibody [NBP1-85806] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PCMT1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCMT1 Antibody [NBP1-85806] - Staining of human stomach shows cytoplasmic positivity in glandular cells.