NBP1-85806

antibody from Novus Biologicals

Targeting: PCMT1

Immunocytochemistry

Antibody data

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Validation data

Product number: NBP1-85806 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NBP1-85806, RRID:AB_11035901
Product name: Rabbit Polyclonal PCMT1 Antibody
Antibody type: Polyclonal
Description: Immunogen affinity purified. Specificity of human PCMT1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 0.1 ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

PCMT1 protein structure - NBP1-85806 shown in red.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: PCMT1 Antibody [NBP1-85806] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells). Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: PCMT1 Antibody [NBP1-85806] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human Cerebral Cortex tissue

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: PCMT1 Antibody [NBP1-85806] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: PCMT1 Antibody [NBP1-85806] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18 Lane 2: Human cell line RT-4

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: PCMT1 Antibody [NBP1-85806] - Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: PCMT1 Antibody [NBP1-85806] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PCMT1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.