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- Antibody Data
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- Validations
- Western blot [6]
- Immunocytochemistry [2]
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- Product number
- GTX629888 - Provider product page
- Provider
- GeneTex
- Product name
- SQSTM1 / P62 antibody [GT239]
- Antibody type
- Monoclonal
- Reactivity
- Human, Rat
- Host
- Mouse
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HepG2 whole cell extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [GT239] (GTX629888) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SQSTM1 antibody [GT239] detects SQSTM1 protein by Western blot analysis.A. 30 ?g A549 whole cell lysate/extractB. 30 ?g H1299 whole cell lysate/extract10 % SDS-PAGESQSTM1 antibody [GT239] (GTX629888) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SQSTM1 antibody detects SQSTM1 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) Huh-7 whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX629888) diluted by 1:1000.The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SQSTM1 antibody detects SQSTM1 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) HepG2 whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX629888) diluted by 1:1000.The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 £gg) was separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [GT239] (GTX629888) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HepG2 whole cell extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [GT239] (GTX629888) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SQSTM1 antibody [GT239] detects SQSTM1 protein at autophagosome by immunofluorescent analysis.Samples: HepG2 cells treated with 3£gM thapsigargin 16 hrs (rigtht) and mock (left) were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 1 min .Green: SQSTM1 protein stained by SQSTM1 antibody [GT239] (GTX629888) diluted at 1:500.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SQSTM1 antibody [GT239] detects SQSTM1 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50£gM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: SQSTM1 protein stained by SQSTM1 antibody [GT239] (GTX629888) diluted at 1:2000.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.
