PA5-35409

antibody from Invitrogen Antibodies

Targeting: SQSTM1 A170, OSIL, p60, p62, p62B, PDB3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-35409

Provider

Invitrogen Antibodies

Product name

Phospho-SQSTM1 (Ser28) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with non-human primate based on 100% sequence homology.

Concentration

~0.25 mg/mL

References

Submitted references

Ketohexokinase-A acts as a nuclear protein kinase that mediates fructose-induced metastasis in breast cancer.

Kim J, Kang J, Kang YL, Woo J, Kim Y, Huh J, Park JW
Nature communications 2020 Oct 28;11(1):5436

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Hyper-phosphorylation of Sequestosome-1 Distinguishes Resistance to Cisplatin in Patient Derived High Grade Serous Ovarian Cancer Cells.

Nguyen EV, Huhtinen K, Goo YA, Kaipio K, Andersson N, Rantanen V, Hynninen J, Lahesmaa R, Carpen O, Goodlett DR
Molecular & cellular proteomics : MCP 2017 Jul;16(7):1377-1392

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-p62 pSer28 in Jurkat lysate using a Phospho-p62 pSer28 polyclonal antibody (Product # PA5-35409). Results show a band at ~48Da. Phosphospecificity is shown in the second lane where immunolabeling is blocked by preadsorption of the phospho-peptide used as antigen but not by the corresponding dephosphopeptide (not shown).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of SQSTM1 in Jurkat cell lysate showing specific immunolabeling of a band at ~48 kDa corresponding to Phospho-SQSTM1 (Ser28) polyclonal antibody (Product # PA5-35409) in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by pre-adsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).